Measurement

Part:BBa_K079026:Design

Designed by: Maria Vittoria Amaduzzi   Group: iGEM08_Bologna   (2008-10-24)


GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1864


Design Notes

Since we had to work with many constructs that have the GFP reporter protein downstream the LacI repressor protein (BBa_C0012) under the control of the Lac promoter, we decided to design this composite part as an expression control. Infact, we espected GFP production to be lower if any Dna sequence was inserted between the promoter and the reporter.


Source

Registry standard parts.

References